Slice And Dice Single Cell Sorting: The Dicer Program

Dicer is a computer program for performing single cell sorting by the "Slice And Dice" method, devised by Geoffrey Osborne at the Queensland Brain Institute, for maximal sampling of the diversity of cellular populations (to see how, go to the method page).  It functions by controlling a cell sorter of the BD^TM inFlux^TM or Jazz^TM series.  A simulator program is also available that simulates Dicer-style sorting while reading data from a pre-recorded (.fcs) data file, thus facilitating evaluation of the method for particular projects. 

The simulator can be downloaded free for permanent use in evaluating the Dicer function.  For trialling the method in a functional system, download a  functional copy of Dicer for a 30 day free trial.  To retain Dicer after the trial, a licence fee is required (see https://eshop.uniquest.com.au/dicer/). 

Features:

• Performs single cell sorting by the "Slice And Dice" method, maximally sampling the diversity of the delineated cells.
• Can also perform conventional single cell sorting for side-by-side comparison.
  
• Handles sorting into a variety of microtitre plates including the common 96-well and Terasaki plates.
  
• Saves a (.fcs) data file containing identifiable data on the cells sorted to each well.  
  

Current distribution versions:

• Simulator version 0009
  
• Functional Dicer version 0055
  

Bleeding edge:

• Functional Dicer version 0058

For more information, see the links below.

Links:

The Dicer method

Download

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