is provided in a spirit of collegiality for testing by
interested parties. It is provided without warranty of any kind
and no claims are made regarding its suitability for any task.
All are Java programs and will run on systems with a recent Java
version installed. Launch the downloaded ".jar" file by
double-clicking. Recent Mac systems may object that the
application is by an "unknown developer", in which case, see the
tips for installation.
WARNING: Do not subject primary data to these programs. All
files should be backed up prior to processing.
The Cellular Symphony is an
audio representation of flow cytometric data. Each cell is
represented by a musical note of timbre, pitch and volume
dependent on its FCM parameters. The program correlates the
audio output with 2D and 3D graphical display. It may be that
only a musical savant can detect the intricacies in a
3-instrument cellular symphony or it may be that the average
cytometrist also can do so with practice. Your comments are
Note: in addition to Java,
CellSymphony requires an accessible midi synthesizer (supplied
with most modern computers).
Try the example
music clip from a 3-colour data source:
it is feared that a flow cytometry data file has been violated,
for example at the start of the file by cells carried over from
the previous sample, or at the end of the file by a nozzle
blockage, Hackit can be used to clip the offending stream of
cells from the file. It may be used also to concatenate the data
from a number of files.
Where flow cytometry data files
from the clinic are to be used in research projects, DeID is
used to remove patient identification. This is achieved by
writing a copy of the data file with selected keyword values
removed. DeID has been revised (July 2013) to enable
processing of multi-block data files (Beckman-Coulter .LMD, *Guava
microtitre data, *Cell-Dyn